Exploring the Anticancer Potential of Ferula assa-foetida Bioactive Compounds and Molecular Mechanisms in Breast Cancer Treatment
DOI:
Abstract
Breast cancer is a major global health issue, with limited treatment options that often come with adverse side effects. Ferula assa-foetida, a medicinal plant known for its anti-inflammatory, antioxidant, and antimicrobial properties, has shown potential as an anticancer agent. This study investigates the anticancer effects of Ferula assa-foetida extracts on breast cancer cell lines (MCF-7 and T47D). The research identifies key bioactive compounds, such as ferulic acid and flavonoids, and explores their molecular mechanisms. In vitro assays, including cytotoxicity assays (MTT and Alamar Blue) and apoptosis detection (Annexin V/PI staining), were conducted to measure cell proliferation and apoptosis induction. Furthermore, PCR and Western blot analyses were used to examine the expression of apoptosis-related genes (p53, Bcl-2, and caspase-3). The results indicate that Ferula assa-foetida extracts significantly inhibit cell growth and induce apoptosis in breast cancer cells. This study provides insights into the potential of Ferula assa-foetida as a complementary treatment for breast cancer and supports further research to evaluate its clinical applicability.
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M. A. Rahaman, A. Kalam, and M. Al-Mamun, “Unplanned urbanization and health risks of Dhaka City in Bangladesh: uncovering the associations between urban environment and public health,” Front. Public Health, vol. 11, 2023.
S. Roy and T. Sowgat, “Dhaka: Diverse, dense, and damaged neighborhoods and the impacts of unplanned urbanisation,” Healthy and Learning Cities and Neighborhood (SHLC), GCRF Centre for Sustainable, 2021.
B. Liu, H. Zhou, L. Tan, et al., “Exploring treatment options in cancer: tumor treatment strategies,” Sig Transduct Target Ther, vol. 9, p. 175, 2024,
U. Anand, A. Dey, A. K. S. Chandel, R. Sanyal, A. Mishra, D. K. Pandey, V. De Falco, A. Upadhyay, et al., “Cancer chemotherapy and beyond: Current status, drug candidates, associated risks and progress in targeted therapeutics,” Genes Dis., vol. 10, no. 4, pp. 1367-1401, 2022.
M. Zawrzykraj, M. Deptuła, K. Kondej, A. Tymińska, and M. Pikuła, “The effect of chemotherapy and radiotherapy on stem cells and wound healing. Current perspectives and challenges for cell-based therapies,” Biomed. Pharmacother., vol. 168, 2023.
B. K. Prasanth, S. Alkhowaiter, G. Sawarkar, B. D. Dharshini, and A. R. Baskaran, “Unlocking Early Cancer Detection: Exploring Biomarkers, Circulating DNA, and Innovative Technological Approaches,” Cureus, vol. 15, no. 12, 2023.
S. Das, M. K. Dey, R. Devireddy, and M. R. Gartia, “Biomarkers in cancer detection, diagnosis, and prognosis,” Sensors, vol. 24, no. 1, 2024.
M. W. Alruways, “Antimicrobial effects of Ferula species- an herbal tactic for management of infectious diseases,” J. King Saud Univ. Sci., vol. 35, no. 7, 2023.
N. Elarabany, A. Hamad, and N. M. Alzamel, “Antitumor and phytochemical properties of Ferula assa-foetida L. oleo-gum–resin against HT-29 colorectal cancer cells in vitro and in a xenograft mouse model,” Molecules, vol. 28, no. 24, 2023.
R. Kumar, N. Yadav, A. Rana, R. Chauhan, S. Singh, D. Kumar, S. Singh, and A. Kumar, “Ferula assa-foetida L., an important Central and South Asian traditional spice and medicinal herb: A comprehensive review,” J. Appl. Res. Med. Aromat. Plants, vol. 41, 2024.
S. A. A. Rizvi, G. P. Einstein, O. L. Tulp, F. Sainvil, and R. Branly, “Introduction to traditional medicine and their role in prevention and treatment of emerging and re-emerging diseases,” Biomolecules, vol. 12, no. 10, 2022,
Z. Rezaei, M. R. Afshar, and K. Soltani, “Phytochemicals of Ferula assa-foetida with anticancer potential: Molecular insights,” Journal of Traditional and Complementary Medicine, vol. 13, no. 3, pp. 205–215, 2024.
N. Elarabany, A. Hamad, and N. M. Alzamel, “Antitumor and phytochemical properties of Ferula assa-foetida L. oleo-gum–resin against HT-29 colorectal cancer cells in vitro and in a xenograft mouse model,” Molecules, vol. 28, no. 24, 2023.
P. P. Singh, R. Joshi, R. Kumar, A. Kumar, and U. Sharma, “Comparative phytochemical analysis of Ferula assa-foetida with Ferula jaeschkeana and commercial oleo-gum resins using GC–MS and UHPLC-PDA-QTOF-IMS,” Food Res. Int., vol. 164, 2023.
X. K. Fayzullaeva, “Prospects for the use of stinking ferula (Ferula assa-foetida L.), growing in the Republic of Karakalpakstan,” Int. Multidiscip. J. Res. Dev., vol. 10, no. 12, 2023.
A. Pathak, A. P. Gupta, and P. Pandey, “Herbal medicine and sustainable development: Challenges and opportunities,” in Herbal Medicine Phytochemistry, S. C. Izah, M. C. Ogwu, and M. Akram, Eds. Reference Series in Phytochemistry. Springer, 2024.
K. W. A. Lee, L. K. W. Chan, L. C. Hung, P. L. K. W. Phoebe, Y. Park, and K. H. Yi, “Clinical applications of exosomes: A critical review,” Int. J. Mol. Sci., vol. 25, no. 14, 2024.
M. Oliveira, W. Antunes, S. Mota, Á. Madureira-Carvalho, R. J. Dinis-Oliveira, and D. D. da Silva, “An overview of the recent advances in antimicrobial resistance,” Microorganisms, vol. 12, no. 9, 2024.
M. Boța, L. Vlaia, A.-R. Jîjie, I. Marcovici, F. Crişan, C. Oancea, C. A. Dehelean, T. Mateescu, and E.-A. Moacă, “Exploring synergistic interactions between natural compounds and conventional chemotherapeutic drugs in preclinical models of lung cancer,” Pharmaceuticals, vol. 17, no. 5, 2024.
A. Jenča, D. K. Mills, H. Ghasemi, E. Saberian, A. Jenča, A. M. Karimi Forood, A. Petrášová, J. Jenčová, Z. Jabbari Velisdeh, H. Zare-Zardini, and M. Ebrahimifar, “Herbal therapies for cancer treatment: A review of phytotherapeutic efficacy,” Biologics, vol. 18, pp. 229-255, 2024.
K. Elumalai, S. Srinivasan, and A. Shanmugam, “Review of the efficacy of nanoparticle-based drug delivery systems for cancer treatment,” Biomed. Technol., vol. 5, pp. 109-122, 2024
L. C. Liu, Y. H. Chen, and D. W. Lu, “Overview of recent advances in nano-based ocular drug delivery,” Int. J. Mol. Sci., vol. 24, no. 20, 2023.
U. Kaushal, M. Kaur, M. Nagpal, M. Bhuyan, and K. P. Gounder, “Nanocarriers based ocular therapeutics: Updates, challenges and future prospectives,” Curr. Drug Res. Rev., vol. 15, pp. 15-28, 2023.
